Interaction of TiO2 nanoparticles with lung fluid proteins and the resulting macrophage inflammatory response.

TitleInteraction of TiO2 nanoparticles with lung fluid proteins and the resulting macrophage inflammatory response.
Publication TypeJournal Article
Year of Publication2023
AuthorsKM Poulsen, MC Albright, NJ Niemuth, RM Tighe, and CK Payne
JournalEnviron Sci Nano
Volume10
Issue9
Start Page2427
Pagination2427 - 2436
Date Published09/2023
Abstract

Inhalation is a major exposure route to nanoparticles. Following inhalation, nanoparticles first interact with the lung lining fluid, a complex mixture of proteins, lipids, and mucins. We measure the concentration and composition of lung fluid proteins adsorbed on the surface of titanium dioxide (TiO2) nanoparticles. Using proteomics, we find that lung fluid results in a unique protein corona on the surface of the TiO2 nanoparticles. We then measure the expression of three cytokines (interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and macrophage inflammatory protein 2 (MIP-2)) associated with lung inflammation. We find that the corona formed from lung fluid leads to elevated expression of these cytokines in comparison to bare TiO2 nanoparticles or coronas formed from serum or albumin. These experiments show that understanding the concentration and composition of the protein corona is essential for understanding the pulmonary response associated with human exposure to nanoparticles.

DOI10.1039/d3en00179b
Short TitleEnviron Sci Nano